{ "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "FrameKey-0-0-0": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -25, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "642a8d5e-8110-4a0f-88b5-cc31fc6c157b", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1167227, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 145.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:43:45 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-1": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -22, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "e61f77cd-d7d2-4cb1-8aa5-6a01462bb6b4", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1167737, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 148.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:43:46 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-2": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -19, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "a59b3404-17eb-4e5d-a4b7-5c503a127a56", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1168262, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 151.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:43:46 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-3": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -16, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "9296f130-036c-4b99-9851-9da5cc22aa74", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1168771, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 154.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:43:47 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-4": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -13, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "73584ec7-669f-4eac-9590-5dd396009f4d", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1169286, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 157.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:43:47 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-5": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -10, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "65afe68f-dbb5-4242-bf60-4a3783946e0d", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1169813, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 160.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:43:48 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-6": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -7, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "b84b11f8-3af6-4a98-8e9f-bbe013c641dc", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1170340, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 163.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:43:48 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-7": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -4, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "8399d4bd-4910-4e2a-8ab0-0626d2127bdb", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1170885, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 166.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:43:49 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-8": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -1, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "de4b153e-fb88-447b-8d88-01c9983d3303", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1171493, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 169.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:43:49 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-9": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 2, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "ba03456d-fde7-4baa-9c79-b0118c032797", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1172091, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 172.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:43:50 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-10": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 5, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "ba4d169c-80d7-4296-b1b8-c2786e03dc7e", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1172703, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 175.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:43:51 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-11": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 8, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "de557e1c-ebc8-412d-bb37-4df1775dce55", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1173241, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 178.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:43:51 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-12": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 11, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "90c97821-df32-435b-8575-48ce0d86bee0", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1173757, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 181.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:43:52 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-13": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 14, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "e4df5aaf-b414-47f8-be28-a9f231986cc0", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1174271, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 184.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:43:52 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-14": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 17, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "e3e74881-663e-4650-9d94-f05fa82b56c1", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1174783, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 187.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:43:53 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-15": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 20, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "3529ec75-912f-4732-9fe2-a63406aac0a4", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1175294, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 190.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:43:53 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-16": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 23, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "b3b35724-0990-4920-bbbf-5b920594836d", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1175813, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 193.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:43:54 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-0": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -25, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "92815d99-28d4-41d9-a7f4-bc2d295d0821", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1177006, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 145.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:43:55 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-1": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -22, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "dd47b8a0-aaf5-4b20-9236-95bf2ba83df8", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1177314, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 148.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:43:55 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-2": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -19, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "daee3833-a5ac-4819-984d-7b72e1b10c73", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1177623, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 151.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:43:55 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-3": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -16, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "4da17b19-7063-409d-b72b-de09e611aabf", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1177944, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 154.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:43:56 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-4": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -13, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "38132ecf-39cf-4292-89d2-9eee80bd2b16", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1178257, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 157.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:43:56 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-5": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -10, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "130de63a-94d5-4cf3-8d2a-95c8e5822765", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1178563, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 160.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:43:56 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-6": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -7, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "544bf9bd-c53b-4518-8c3a-19c376fec9f7", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1178881, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 163.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:43:57 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-7": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -4, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "7aafcd91-32e2-4874-9a2d-51d595b78173", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1179208, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 166.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:43:57 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-8": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -1, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "f796b82d-a261-4d54-9656-99b49176e5ea", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1179520, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 169.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:43:57 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-9": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 2, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "4326603f-9139-4576-92ec-3ebb660d4a30", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1179883, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 172.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:43:58 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-10": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 5, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "71c02352-c91c-4c21-adec-828403de5045", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1180191, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 175.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:43:58 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-11": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 8, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "b9304f41-c72c-4c3a-8770-2be6d695ec54", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1180510, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 178.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:43:58 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-12": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 11, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "02f95f9e-8239-4fb3-b1e3-fec35826749d", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1180822, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 181.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:43:59 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-13": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 14, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "a03a2796-069c-4c3a-824c-90f502ec5f5a", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1181181, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 184.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:43:59 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-14": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 17, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "19a29532-44af-4f07-b70d-0a700ed333a7", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1181485, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 187.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:43:59 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-15": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 20, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "43d6bd05-5be0-4c32-b8b7-f4cc7551a570", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1181819, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 190.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:44:00 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-16": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 23, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "bd3ff09c-293b-49c4-863b-1047c6a13baf", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1182130, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 193.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:44:00 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-0": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -25, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "165bebee-0ffd-4e26-955d-8e26cdea4850", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1182966, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 145.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:44:01 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-1": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -22, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "660c1218-5ed4-450f-8b68-e4742caf0bab", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1183434, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 148.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:44:01 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-2": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -19, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "179db882-43cd-4377-bf0c-a16a3c3507e7", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1183858, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 151.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:44:02 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-3": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -16, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "de02c45b-91ff-4f3d-9172-5e0274941215", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1184222, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 154.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:44:02 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-4": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -13, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "9735c16a-06fe-45f8-b159-aac04162ec9f", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1184595, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 157.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:44:02 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-5": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -10, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "d83c5092-9da8-4b2e-8fda-cde7717246ca", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1185117, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 160.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:44:03 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-6": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -7, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "be389d11-1f2f-4dc5-b61d-be9bbeadc386", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1185580, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 163.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:44:03 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-7": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -4, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "3b301176-726d-4296-b0ce-c76e750b3c76", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1185952, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 166.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:44:04 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-8": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -1, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "1a92bb55-328d-44a0-8a87-8e05bafee59c", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1186318, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 169.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:44:04 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-9": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 2, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "c0742c6f-5f7f-4b84-bf22-d896c02f1bd8", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1186690, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 172.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:44:05 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-10": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 5, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "35af3428-a28e-498e-9182-0ce4f6a2333e", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1187050, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 175.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:44:05 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-11": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 8, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "d727432b-79cd-45df-b344-dc38d75f2cf2", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1187423, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 178.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:44:05 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-12": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 11, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "08eba17d-f910-4b07-b49f-2c0109699e3c", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1187786, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 181.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:44:06 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-13": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 14, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "659b5810-52b4-4dfe-8467-93da848a75c8", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1188154, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 184.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:44:06 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-14": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 17, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "1a5dffb4-5511-4b23-b514-095944685b6d", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1188524, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 187.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:44:06 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-15": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 20, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "035ba986-b91f-4e26-8004-37c58ee823e4", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1188888, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 190.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:44:07 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-16": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 23, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "96cb11e5-e50b-41c0-bc04-22874bafa891", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1189263, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 193.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:44:07 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-0": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -25, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "a18ae6ae-2ed7-4ec4-a9d3-f7f365d7071d", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1190037, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 145.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:44:08 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-1": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -22, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "4b5c8db8-11a4-4d0c-aa09-8cd59425152b", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1190581, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 148.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:44:08 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-2": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -19, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "474c0979-8b5c-4a95-baee-ab6bb1d24ff4", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1191194, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 151.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:44:09 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-3": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -16, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "9ba38e3e-0134-4e1b-91bd-6ac2a3cdefc1", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1191679, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 154.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:44:10 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-4": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -13, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "6299377f-3da7-49b9-9531-33f8b59e62e1", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1192142, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 157.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:44:10 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-5": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -10, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "42da3459-aa24-458d-a032-76628b304d14", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1192606, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 160.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:44:10 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-6": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -7, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "61d358fb-c230-47d8-8d3b-51e71354cf43", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1193067, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 163.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:44:11 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-7": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -4, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "3cfb7c9b-e306-47e2-aed2-a4170e7058fa", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1193581, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 166.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:44:11 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-8": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -1, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "85cba60d-0ca1-465a-a25e-dd0f106c0e61", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1194045, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 169.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:44:12 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-9": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 2, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "c1cd4f90-e449-415d-89bc-07ee6fed8644", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1194537, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 172.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:44:12 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-10": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 5, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "c6d6244d-01d6-4180-850d-893a13219c62", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1195003, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 175.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:44:13 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-11": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 8, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "de6a0904-602e-419b-b177-c28e837390e7", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1195467, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 178.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:44:13 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-12": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 11, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "ae9accb1-277f-4a9f-b4b4-01c67c8a150a", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1195944, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 181.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:44:14 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-13": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 14, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "362ce2f3-8099-4a1a-9d38-ba4c5a5d28a1", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1196425, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 184.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:44:14 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-14": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 17, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "6061a8fb-d885-4a60-a822-087d1b1b7738", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1197088, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 187.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:44:15 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-15": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 20, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "131be2e8-9376-4a73-b332-66b659f08483", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1197543, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 190.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:44:15 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-16": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 23, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "e3523642-b850-4554-b647-186c78fe93a1", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 37, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:43:45 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 37, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1198031, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Thalamus_03", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -44296.1, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 193.02, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:44:16 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1698, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" } }