{ "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "FrameKey-0-0-0": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -25, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "576d4d45-0cf5-44c0-a392-170d93fcb978", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1479162, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 145.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:48:57 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-1": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -22, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "41d7d74b-ca37-4546-ac0b-9b49e5fc46e5", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1479671, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 148.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:48:57 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-2": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -19, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "5bbc3d50-0efd-4461-bc75-f0efece66731", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1480189, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 151.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:48:58 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-3": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -16, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "1f072fe3-0e9f-45a2-b7b4-bf46d259db2e", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1480699, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 154.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:48:59 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-4": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -13, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "b67dbd0f-a848-4384-b3a6-905b70f41d01", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1481206, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 157.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:48:59 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-5": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -10, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "9ac4e9ed-ecf1-44e7-af7d-5efbe5cfb5c6", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1481718, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 160.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:00 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-6": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -7, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "5fdd2b07-0201-4e2e-abf8-ac7245aa2fcd", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1482238, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 163.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:00 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-7": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -4, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "5ca7f670-45cb-410e-bf6c-57a47b71ad78", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1482756, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 166.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:01 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-8": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -1, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "9cddf208-4b8b-40cb-8636-b9707b12da8d", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1483268, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 169.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:01 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-9": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 2, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "0f646133-f4fc-447c-b13a-dbd7de401664", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1483779, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 172.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:02 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-10": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 5, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "ebd75b5a-277d-46a1-a81d-61c57efafd12", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1484290, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 175.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:02 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-11": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 8, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "c59c60d9-99f5-4ea0-a267-9898d80413df", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1484802, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 178.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:03 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-12": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 11, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "d99f472c-8b38-45c3-b262-7c1d3b167cb3", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1485314, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 181.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:03 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-13": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 14, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "b65cd7b5-25f1-4a78-bb6b-d620b8d48542", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1485825, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 184.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:04 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-14": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 17, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "8eeda509-3232-4912-8ff4-9f434534a3cb", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1486338, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 187.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:04 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-15": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 20, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "fd40277c-e945-4992-9c63-d40cb4b0106d", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1486853, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 190.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:05 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-16": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 23, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "c0f85d66-3cf2-4e07-94e4-208495786e48", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1487380, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 193.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:05 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-0": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -25, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "ef99f252-6ada-4ee3-8de2-bc2edd005ef1", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1488576, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 145.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:06 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-1": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -22, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "adbe0678-b48c-42f9-8aaf-f779171e42fa", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1488890, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 148.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:07 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-2": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -19, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "d10e8060-17ec-4652-ad3d-6ff96628d7b3", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1489205, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 151.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:07 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-3": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -16, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "6c00a9b6-8d40-47d9-aa06-64fc899e3264", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1489509, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 154.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:07 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-4": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -13, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "ce6e49a6-fb20-4afd-88f8-644771432131", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1489818, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 157.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:08 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-5": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -10, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "88b7a6cf-24a7-4293-95a1-5879a70ba2ec", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1490132, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 160.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:08 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-6": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -7, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "1a70f79e-e736-4b0f-981e-11823728aa3f", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1490444, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 163.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:08 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-7": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -4, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "7e70416c-c87c-47f5-b366-40e277cdf1c4", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1490759, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 166.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:09 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-8": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -1, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "54c39151-d7fa-4922-bc3e-589bc0f1464b", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1491077, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 169.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:09 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-9": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 2, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "94872333-718b-4df7-a707-1a66285ac20d", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1491397, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 172.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:09 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-10": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 5, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "ba56695a-0d68-45a5-9196-109e18ddeccc", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1491701, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 175.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:10 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-11": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 8, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "d549d7b8-5e12-4388-87bc-bb3860fb3524", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1492020, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 178.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:10 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-12": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 11, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "6a94a6b7-0af7-430f-8c18-0e1c257575e9", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1492339, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 181.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:10 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-13": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 14, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "3b5e6fdc-0027-4a06-b440-94950ff96ac6", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1492644, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 184.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:10 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-14": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 17, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "8412e604-c3b1-4037-a1d3-9eadfda3219c", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1492963, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 187.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:11 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-15": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 20, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "dda93b87-729b-4318-b444-775153dcdcd7", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1493283, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 190.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:11 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-16": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 23, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "ed88ec1c-c0e6-44a8-a797-cd27ab6ce985", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1493590, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 193.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:11 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-0": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -25, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "88028eff-4213-44b3-bf32-9ff5935d63f4", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1494281, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 145.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:12 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-1": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -22, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "1bd9f5d1-7cbd-4d49-a04b-fb149abe4e98", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1494647, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 148.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:12 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-2": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -19, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "d51a5409-d59e-4466-964d-7099f602ec2e", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1495016, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 151.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:13 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-3": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -16, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "e4d665e2-8c51-45f8-a71c-96d03a5c03a7", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1495377, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 154.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:13 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-4": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -13, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "a3594ff7-e754-4f1a-b101-568710966105", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1495735, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 157.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:14 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-5": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -10, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "9f79247a-9b46-484d-852f-0e22103e0579", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1496102, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 160.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:14 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-6": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -7, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "58686e1c-8b6e-47ba-a3ea-86e66d0fd605", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1496464, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 163.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:14 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-7": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -4, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "6f585a64-01bc-450f-937b-4dafe3c41163", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1496817, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 166.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:15 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-8": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -1, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "404cc4fb-e4a7-4f1a-90a1-27ea195944af", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1497185, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 169.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:15 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-9": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 2, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "d58796cb-2525-47d2-aa8d-6e6baf7afc3f", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1497551, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 172.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:15 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-10": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 5, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "bb7bd25a-2766-43d8-8be3-74cd64c98470", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1497903, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 175.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:16 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-11": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 8, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "a995116f-b703-4831-aca3-7fa671f6d35b", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1498255, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 178.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:16 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-12": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 11, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "3ce30a27-45a3-4cc5-af37-7d479efc0114", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1498623, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 181.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:16 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-13": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 14, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "61e0e82f-15f0-494f-a16b-ed63810c07fe", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1498996, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 184.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:17 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-14": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 17, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "f0490e18-69e5-42df-972a-d75b67ed56fd", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1499357, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 187.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:17 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-15": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 20, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "af6a8608-aaf7-41c6-9958-d8ac396b2ec6", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1499710, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 190.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:18 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-16": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 23, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "1602d7f5-0d46-446c-b278-52667345fcf2", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1500081, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 193.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:18 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-0": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -25, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "794fcae5-aa76-477b-8485-800f29492016", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1500852, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 145.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:19 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-1": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -22, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "5027f2d6-41e2-4ae7-b9b8-d5afaeb2f8ce", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1501311, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 148.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:19 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-2": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -19, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "9c004ab2-c168-43c6-881d-460c94408ba5", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1501776, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 151.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:20 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-3": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -16, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "6831800f-feb2-462a-a236-49e4bf3c6ec0", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1502240, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 154.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:20 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-4": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -13, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "51a47643-3992-436b-b3f0-778b75923673", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1502704, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 157.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:21 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-5": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -10, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "b009d81a-0e0f-4db8-9d0b-f8c142abc184", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1503166, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 160.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:21 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-6": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -7, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "a7ff437f-8534-4eb3-b4d2-d9117eb24848", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1503636, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 163.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:21 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-7": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -4, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "f57d82cf-d8a2-47d7-b8e4-13ce8a3e5ec0", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1504098, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 166.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:22 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-8": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -1, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "2461e02b-61fa-4fc0-858f-7650a0e1c9e8", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1504557, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 169.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:22 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-9": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 2, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "c8ff128b-74c9-4786-81a5-f57affc01174", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1505022, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 172.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:23 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-10": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 5, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "bb23459f-41b4-429c-b6bc-115d3fa1942c", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1505485, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 175.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:23 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-11": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 8, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "1c6429f8-d92b-44d0-854b-77f8ef9d834c", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1505948, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 178.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:24 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-12": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 11, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "3eea94bb-9aec-4d8a-b6a1-2821d82978ba", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1506411, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 181.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:24 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-13": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 14, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "207c775d-2068-4481-8c31-73b35fcc1900", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1506876, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 184.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:25 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-14": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 17, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "493ab600-86f4-4deb-b5c8-ba366f068354", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1507339, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 187.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:25 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-15": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 20, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "d65ad49c-a28a-4989-a1fa-0848b657a75a", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1507804, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 190.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:26 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-16": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 23, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "226b1d76-7fd5-47a2-bdf7-c2675cd05c10", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 47, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:48:57 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 47, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 1508270, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -38705.3, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 193.005, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:49:26 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -1261.7, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" } }