{ "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "FrameKey-0-0-0": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -25, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "a6f5e878-f8b4-46fc-98d5-234966a34ccf", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 221129, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 145.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:27:59 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-1": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -22, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "5240e51a-f161-44fb-9edd-1d1afc4ac8df", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 221672, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 148.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:27:59 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-2": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -19, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "1393c0eb-df0a-45d2-90dc-d6bbd0ea03a5", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 222194, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 151.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:00 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-3": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -16, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "4b4743a7-2674-47ac-9927-181252cd090f", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 222707, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 154.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:01 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-4": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -13, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "bd08b5af-bb04-41c7-b4dc-9effcf77eb40", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 223241, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 157.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:01 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-5": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -10, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "857a5f05-c159-4246-982c-03d3604cfb00", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 223763, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 160.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:02 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-6": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -7, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "e2ef8716-be54-41f0-ac5c-76ec6bd039bd", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 224272, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 163.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:02 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-7": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -4, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "31fc4c3d-68f8-43eb-b83b-f479f0f6a894", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 224784, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 166.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:03 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-8": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -1, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "5a7236b4-5500-473f-988c-c275a9e93c30", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 225294, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 169.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:03 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-9": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 2, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "6fa729ff-89ca-4b73-970e-00c59606c512", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 225805, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 172.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:04 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-10": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 5, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "3a55b69f-555c-4112-aa1a-f82decb4eb6e", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 226316, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 175.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:04 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-11": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 8, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "003a6d87-4a5a-4552-91f8-ef782c7fe7d1", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 226828, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 178.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:05 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-12": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 11, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "fe747a34-5e77-4d2c-a51c-68a504a4bcea", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 227340, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 181.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:05 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-13": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 14, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "c94bb228-2bd7-43ca-a097-eb8e5041f20b", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 227851, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 184.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:06 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-14": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 17, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "59f53af4-b557-4362-95da-1f883653b30e", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 228363, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 187.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:06 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-15": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 20, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "d65be0dc-5e05-4931-9eb4-59e9e07f07f6", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 228874, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 190.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:07 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-16": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 23, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "689d8085-1702-4d95-997d-2cf54aa7e5cc", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 229390, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 193.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:07 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-0": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -25, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "c7146687-be21-4e53-a17e-ac99a33f3992", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 230604, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 145.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:08 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-1": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -22, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "5b5432b1-a98b-4e25-9f25-050f82833df5", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 230912, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 148.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:09 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-2": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -19, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "4326d74c-7be8-4306-952f-dd08cbdba450", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 231215, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 151.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:09 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-3": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -16, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "67ad6355-cb2c-40f1-94de-a69245f78144", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 231520, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 154.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:09 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-4": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -13, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "1b64d916-0556-4e21-b9e3-201a1699046a", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 231839, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 157.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:10 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-5": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -10, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "3b9fbbab-889f-4b02-ae6c-9092e009be17", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 232144, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 160.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:10 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-6": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -7, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "19461ac1-b424-462a-ab86-0871f00d88ae", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 232463, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 163.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:10 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-7": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -4, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "209d1c69-6160-453a-851a-c7b88df37830", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 232765, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 166.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:11 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-8": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -1, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "fe06aa56-92d9-45f8-a7f6-3c4d38ec3130", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 233082, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 169.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:11 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-9": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 2, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "3bb2da0e-5c95-4f6b-91c9-b075fccbae8c", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 233391, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 172.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:11 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-10": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 5, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "35404ffa-2785-4038-972b-ed9ea8219b45", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 233711, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 175.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:12 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-11": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 8, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "33ecf295-f6bd-428b-8b90-379c03d7e5ab", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 234017, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 178.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:12 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-12": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 11, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "ce8d0560-5c5a-415e-b32b-f435bc7df5f6", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 234333, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 181.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:12 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-13": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 14, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "59e3063c-b498-4f63-bbeb-55cfecd97b93", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 234636, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 184.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:12 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-14": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 17, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "680d3943-3c14-4276-82f7-11098d31b2e1", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 234939, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 187.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:13 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-15": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 20, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "4f32f9f1-7030-488a-8ff4-418a921a6238", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 235261, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 190.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:13 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-16": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 23, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "2483d4a3-c083-45e8-94ea-1009994e34a1", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 235584, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 193.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:13 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-0": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -25, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "e7d6e38f-0de5-4799-9a7a-52403bf1c47d", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 236277, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 145.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:14 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-1": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -22, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "40d4580d-3971-4368-8a27-8c59f32d5698", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 236638, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 148.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:14 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-2": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -19, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "1b8a4a90-3009-47d6-9f23-dcdb02555e6a", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 237004, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 151.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:15 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-3": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -16, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "a0f144d9-71a0-4d7b-a361-4a27577357d4", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 237371, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 154.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:15 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-4": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -13, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "3bf886c9-ba47-4174-bc27-07b9eb177bd1", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 237738, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 157.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:16 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-5": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -10, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "87460641-f98f-4c62-99f8-a45ed6e0a8a0", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 238106, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 160.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:16 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-6": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -7, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "f92d4f25-96f0-43be-a436-9573c17c680f", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 238458, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 163.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:16 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-7": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -4, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "a2e22127-198e-4140-8661-704d6530bb15", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 238810, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 166.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:17 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-8": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -1, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "652e3d0e-8546-413f-a85b-d4b143eb824f", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 239183, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 169.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:17 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-9": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 2, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "288ed8b1-ac86-41bb-b217-7086acd7578e", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 239547, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 172.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:17 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-10": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 5, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "77ebc045-6ebd-4ac2-995b-b36c27454740", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 239915, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 175.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:18 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-11": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 8, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "8a69d060-25f0-409f-b5e4-9bded7306bc5", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 240283, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 178.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:18 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-12": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 11, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "466f3b9f-826c-4199-96ad-b3c70d1f3e33", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 240648, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 181.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:18 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-13": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 14, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "76f2c283-b922-4de8-b1ac-90bf2db2f692", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 241018, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 184.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:19 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-14": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 17, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "d6ca2b37-29da-4268-b35e-ea49b6825a00", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 241385, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 187.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:19 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-15": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 20, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "27a4fa16-f7a0-4e1e-8195-9ba47f269ee3", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 241752, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 190.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:20 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-16": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 23, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "51bd66c2-7ecc-43fa-aeea-5af1453dd0c8", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 242124, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 193.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:20 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-0": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -25, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "40e06f50-5661-411d-9517-da82c9145471", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 242914, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 145.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:21 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-1": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -22, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "b38d5d4b-7122-4e38-8aad-fb83061f26b2", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 243372, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 148.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:21 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-2": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -19, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "b7d5d87c-ed93-419c-9fb2-a8d928e5f1e8", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 243833, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 151.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:22 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-3": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -16, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "8f50c2e4-9006-43c7-8867-94388de70ca8", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 244298, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 154.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:22 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-4": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -13, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "da1e5749-82c4-4e71-8702-05e49d36b916", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 244761, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 157.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:23 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-5": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -10, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "6a0f7763-a5ee-498c-84f8-149e10d88faf", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 245276, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 160.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:23 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-6": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -7, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "80f6331c-dceb-4338-b824-72eb3668b10e", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 245736, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 163.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:24 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-7": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -4, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "36af4bcc-a071-4e53-a76d-21c62c3ef47e", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 246199, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 166.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:24 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-8": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -1, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "8f918c0c-4135-4506-99ad-e966a4874acc", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 246663, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 169.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:24 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-9": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 2, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "bdf0f1e7-097b-42d4-8f9d-3be686629fdd", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 247131, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 172.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:25 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-10": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 5, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "ebfcdb96-b299-489d-89ae-a15f323f61e6", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 247589, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 175.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:25 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-11": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 8, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "02f8c4e5-c6ce-42a1-9189-286f701ccf59", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 248054, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 178.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:26 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-12": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 11, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "7ed886e2-0be0-49f5-8319-ca3341ef79a7", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 248516, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 181.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:26 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-13": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 14, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "05d20347-27d1-4bd3-9d92-aa3352560fc0", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 248980, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 184.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:27 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-14": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 17, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "46cf2738-b751-4c42-aa69-7f0a286f50c5", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 249443, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 187.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:27 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-15": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 20, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "d2cf59cc-7a53-4def-b71f-5050b41d4a52", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 249908, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 190.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:28 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-16": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 23, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "6eba0d44-ea98-4fe4-a9f3-25deead6ff1b", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 7, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 00:27:59 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 7, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 250377, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide40L_Seq03_Injection_08", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -46796.2, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 193.025, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 00:28:28 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": 5446.1, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" } }