{ "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "FrameKey-0-0-0": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -25, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "2f5ad591-31d6-45a1-ab06-028b9204fba8", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2317801, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 144.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:02:56 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-1": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -22, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "ba4b61cc-f6a4-44c5-8c19-b7c79ee70675", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2318420, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 147.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:02:56 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-2": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -19, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "6107728f-30e7-4300-b660-af61c92714cc", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2318951, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 150.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:02:57 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-3": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -16, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "5baf4485-9543-40ea-98f7-0382620f4e04", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2319501, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 153.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:02:57 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-4": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -13, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "94d1f204-32f8-4c16-b8eb-189e6c925058", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2320035, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 156.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:02:58 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-5": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -10, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "de4196d6-3b89-452b-ae19-cbc780b601bd", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2320576, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 159.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:02:58 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-6": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -7, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "1cfb96ea-52cf-4d77-a8bc-ac8ea72a4d85", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2321167, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 162.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:02:59 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-7": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -4, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "6d8fdbc6-cfdb-473f-bc84-e189cc7c62cc", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2322230, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 165.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:03:00 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-8": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -1, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "8a2dc752-a5de-4893-88bb-5cbb929d5592", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2322767, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 168.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:03:01 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-9": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 2, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "a677c7b8-2b61-4fe3-adba-4048b0c0d32b", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2323304, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 171.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:03:01 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-10": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 5, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "c0858599-7402-472c-af55-612d7931e09c", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2323840, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 174.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:03:02 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-11": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 8, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "b96ee41a-b53c-4ed7-b917-2ad3e81df16d", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2324381, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 177.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:03:02 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-12": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 11, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "13e36ea7-c26a-4a22-ae90-c1dacd45c8ae", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2324922, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 180.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:03:03 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-13": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 14, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "b29bfd70-1a23-4b52-8517-7cba83aa71a4", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2325470, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 183.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:03:03 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-14": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 17, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "878db4dd-8631-411b-811f-d895bb41de92", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2326001, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 186.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:03:04 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-15": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 20, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "1", "PRIME-FullWellCapacity": "62000", "UUID": "c8adcbc8-1109-4840-8772-6dcd2ddb9285", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2326543, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 189.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:03:04 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-0-16": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 23, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 300, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "d9c09cd7-f8bd-47d6-828a-fd59e78bd9f1", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "565/24(G)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "300.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2327075, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 192.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:03:05 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-0": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -25, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "fcfb2833-c670-446a-8823-1d91b64fbb12", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2328316, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 144.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:03:06 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-1": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -22, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "5cbc64a1-9f88-43bf-a264-02e607d209d7", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2328650, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 147.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:03:06 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-2": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -19, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "531d4e4e-50d6-41bf-99e3-6cda5db4340b", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2328990, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 150.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:03:07 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-3": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -16, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "138d37c0-f8d5-429a-9b76-1304429ecaad", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2329386, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 153.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:03:07 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-4": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -13, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "7d0bc31a-fd91-4784-b7b2-324bf46f7af6", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2329720, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 156.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:03:08 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-5": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -10, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "a8b0f49b-f7a1-422a-8963-0f07609b3d58", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2330056, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 159.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:03:08 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-6": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -7, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "5e19e7fc-6cf3-48ca-a370-efab3716c978", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2330384, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 162.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:03:08 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-7": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -4, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "772a6374-f67a-4d9c-aaf7-67c6a2c6372a", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2330754, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 165.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:03:09 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-8": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -1, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "c480809b-c970-4768-b6f2-88f891045b15", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2331116, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 168.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:03:09 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-9": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 2, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "a39a26ef-842b-4447-bd33-86188fba8b97", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2331461, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 171.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:03:09 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-10": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 5, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "900ec0cb-2a2f-4de8-a945-0877a06e446c", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2331802, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 174.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:03:10 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-11": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 8, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "d50d46fc-9623-4c53-976e-e0ae6cac8287", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2332194, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 177.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:03:10 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-12": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 11, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "18f703af-d3ad-4f13-9cb7-dd88b0e5aee1", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2332535, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 180.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:03:10 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-13": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 14, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "ec3b428f-f6cc-4305-9d7a-4db6974b7e08", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2332867, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 183.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:03:11 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-14": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 17, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "65afcfe8-0592-4607-b735-6226ca6475c3", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2333207, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 186.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:03:11 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-15": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 20, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "45882e18-4065-4df8-ba8d-55bae1ec146f", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2333536, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "1", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 189.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:03:11 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-1-16": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 23, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 100, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "e4c133e7-de33-4a01-b389-5f9268644a18", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "585/11(T)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "100.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2333890, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 192.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:03:12 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "0.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "5.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-0": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -25, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "0531dd4a-56bd-48b0-b68c-697da42af18c", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2334813, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 144.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:03:13 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-1": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -22, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "579e7346-6057-4522-8b65-f9503f90f025", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2335309, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 147.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:03:13 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-2": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -19, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "2348e75d-2f8a-4162-8fe7-ccb8e36dc1c5", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2335681, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 150.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:03:14 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-3": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -16, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "594b25fa-53f5-4d87-8e8e-bd0db366e217", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2336066, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 153.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:03:14 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-4": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -13, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "4fa010f7-bd33-4336-ac8b-b6d61e8529b6", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2336468, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 156.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:03:14 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-5": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -10, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "151d145f-31ee-4559-b255-d7f199f1937f", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2336866, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 159.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:03:15 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-6": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -7, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "66e2fc62-5e28-4eb4-87e4-6ab2fb67c6c6", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2337265, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 162.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:03:15 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-7": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -4, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "f35db87a-605e-4384-942b-2d224727d4f0", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2337682, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 165.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:03:16 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-8": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -1, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "27deb29f-d0d5-4f2e-80f7-425c8ac1e1f9", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2338084, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 168.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:03:16 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-9": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 2, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "47fedbe8-0666-4b24-bb54-203afa6f0cef", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2338466, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 171.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:03:16 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-10": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 5, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "8fc4388d-fadc-4863-85ae-0f9259c9b15f", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2338853, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 174.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:03:17 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-11": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 8, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "a1f9189e-3182-4f13-97ad-4352685599e7", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2339235, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 177.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:03:17 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-12": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 11, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "cbc4d8ff-0809-4ed4-ad48-2430cb1431e0", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2339621, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 180.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:03:17 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-13": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 14, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "1159a053-529f-4849-90da-3f74d2283798", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2340015, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 183.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:03:18 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-14": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 17, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "1152d8ec-171a-47e3-b759-8ca8217e48fb", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2340391, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 186.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:03:18 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-15": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 20, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "6b00e633-cad1-4493-83ab-4e4331356fe2", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2340763, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 189.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:03:19 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-2-16": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 23, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 150, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "3b834169-8112-4ee3-af20-7cc677cc1b55", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "679/29 (A)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "150.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2341158, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 192.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:03:19 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-0": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -25, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "b1cdec21-bf4f-4a10-98ca-778662f1699a", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2341956, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 144.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:03:20 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-1": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -22, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "35e527ba-c905-4a58-be46-06064b5fe4d5", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2342452, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 147.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:03:20 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-2": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -19, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "53a0bc4c-adcf-451b-b4c1-723584ada0eb", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2343160, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 150.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:03:21 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-3": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -16, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "6eb01994-91e7-49c8-b489-e8dfc20574ce", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2343699, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 153.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:03:22 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-4": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -13, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "e51687cf-3aa8-4044-88cc-d51077558a97", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2344203, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 156.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:03:22 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-5": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -10, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "eeb2e2b3-b546-481a-90c9-62ee27dada7a", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2344683, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 159.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:03:23 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-6": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -7, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "1e492f56-b121-4bd2-b462-ed9b62305102", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2345166, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 162.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:03:23 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-7": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -4, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "ac535247-0530-489e-a9b3-e09d39a1ec2f", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2345698, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 165.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:03:24 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-8": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": -1, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "fbf212c5-18d5-4c6d-876e-8b1bf8b91b36", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2346189, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 168.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:03:24 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-9": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 2, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "92a5b210-fa18-41bd-bad8-9d3f90d71aa9", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2346686, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 171.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:03:25 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-10": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 5, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "0943c5b2-17f4-4ac0-9a86-aeb9a84fe38e", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2347201, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 174.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:03:25 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-11": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 8, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "cc539451-471e-4bb4-9b8b-660b1c0c86e3", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2347706, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 177.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:03:26 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-12": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 11, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "fad15be2-2499-4ad1-83a7-e4453e523b6f", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2348185, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 180.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:03:26 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-13": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 14, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "3ffccc97-12a7-44b7-bd43-83b260b0217d", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2348659, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 183.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:03:26 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-14": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 17, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "4ac2c53c-9268-4da1-9471-59c9e4275f51", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2349151, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 186.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:03:27 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-15": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 20, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "dc23979c-bc56-4b9b-9d11-13f99f374f93", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2350129, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 189.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:03:28 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "1", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" }, "FrameKey-0-3-16": { "Core-Initialize": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "405-DA Device": "405 Shutter", "PRIME-Y-dimension": "1200", "470 Shutter-Block voltage": "false", "PRIME-MetadataEnabled": "No", "555-Name": "DA Shutter", "PRIME-TransposeMirrorX": "0", "PRIME-TransposeMirrorY": "0", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "COM1-Description": "Serial port driver (boost:asio)", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-1200-1200", "520 AO-Description": "NI DAC", "Vincent-D3-Address": "x", "PRIME-PP 5 ENABLED": "No", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "PRIME-Offset": "100", "SlicePosition": 23, "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 250, "405 AO-MaxVolts": "5.0000", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "470 AO-MinVolts": "0.0000", "PRIME-Timing-PostTriggerDelayNs": "24672000.0000", "555 Shutter-MinVolts": "0.0000", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "PRIME-PP 4 ENABLED": "Yes", "PRIME-CCDTemperatureSetPoint": "-15.0000", "COM9-DelayBetweenCharsMs": "0.0000", "PRIME-ChipName": "GS144BSI", "COM1-Parity": "None", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "520 AO-TriggerInputLine": "/Dev1/PFI0", "COM9-BaudRate": "9600", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "Focus-Name": "Focus", "COM1-StopBits": "1", "445 Shutter-IOChannel": "Dev1/ao9", "PIEZO-LudlSingleAxisName": "B", "PRIME-PP 1 ENABLED": "Yes", "640 Shutter-IOChannel": "Dev1/ao14", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FilterBlock-State": "5", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "PRIME-FullWellCapacity": "62000", "UUID": "7e9fe779-bb21-4649-9522-ba98ab515419", "PRIME-ClearMode": "Pre-Exposure", "Core-Camera": "PRIME", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "2", "TE2000-Name": "TE2000", "COM1-Handshaking": "Off", "Core-ChannelGroup": "Channels", "PositionIndex": 73, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos 40um", "555-DA Device": "555 Shutter", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "445 AO-TriggerSampleRate": "100000", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "PRIME-PP 3 THRESHOLD": "80", "520 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 6 Reset": "No", "PRIME-PP 3 ENABLED": "Yes", "Summary": { "Slices": 17, "InitialPositionList": [ { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4324.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4324.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4324.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 4885.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 4885.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 4885.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -47357.2, 5446.1 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46796.2, 5446.1 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19864.3], "PIEZO": [170.025], "XYStage": [ -46235.2, 5446.1 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 3653.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 3653.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -44957.5, 4214.5 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19876.2], "PIEZO": [170.03], "XYStage": [ -45518.5, 4214.5 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 3504.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 3504.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 3504.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4065.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4065.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4065.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -40412.9, 4626.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39851.9, 4626.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19873.1], "PIEZO": [169.89], "XYStage": [ -39290.9, 4626.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40L_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 5630.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 5630.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -44542.5, 6191.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40L_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19893.9], "PIEZO": [169.97], "XYStage": [ -45103.5, 6191.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40L_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -2051 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -2051 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -2051 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -1490 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -1490 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -1490 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46837.1, -929 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -46276.1, -929 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19796.2], "PIEZO": [170.02], "XYStage": [ -45715.1, -929 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -2259 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -2259 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44296.1, -1698 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19798.5], "PIEZO": [170.02], "XYStage": [ -44857.1, -1698 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -2383.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -2383.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -2383.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1822.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1822.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1822.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39827.3, -1261.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -39266.3, -1261.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19823.3], "PIEZO": [170.005], "XYStage": [ -38705.3, -1261.7 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide40R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, -482.3 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, -482.3 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44187.2, 78.7 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide40R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19830.8], "PIEZO": [172.51], "XYStage": [ -44748.2, 78.7 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide40R_Seq03_Visual_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -871.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -871.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -871.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, -310.6 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, -310.6 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, -310.6 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -8264.1, 250.4 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Injection_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7703.1, 250.4 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Injection_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19645], "PIEZO": [169.965], "XYStage": [ -7142.1, 250.4 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Injection_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -1306 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -1306 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -5565.7, -745 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Thalamus_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19651], "PIEZO": [169.98], "XYStage": [ -6126.7, -745 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Thalamus_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -1169.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -1169.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -1169.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_03", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -608.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_04", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -608.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_05", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -608.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_06", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -1144.8, -47.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Contra_07", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -583.8, -47.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Contra_08", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19620.7], "PIEZO": [169.995], "XYStage": [ -22.8, -47.9 ] }, "GridColumnIndex": 2, "Label": "EF65ASlide30R_Seq03_Contra_09", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 638.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_01", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 638.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_02", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5227.3, 1199.9 ] }, "GridColumnIndex": 1, "Label": "EF65ASlide30R_Seq03_Visual_03", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "Focus": [19659.5], "PIEZO": [170.02], "XYStage": [ -5788.3, 1199.9 ] }, "GridColumnIndex": 0, "Label": "EF65ASlide30R_Seq03_Visual_04", "GridRowIndex": 1 } ], "Interval_ms": 0, "UUID": "56e18137-c22c-4681-8653-6dc71860472d", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-06-16 01:02:56 -0400", "Date": "2019-06-16", "z-step_um": 3, "MetadataVersion": 10, "PositionIndex": 73, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0 ], "Width": 1200, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20180219", "ROI": [ 0, 0, 1200, 1200 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 1200, "GridColumn": 0, "Prefix": "Seq03_5", "PixelSize_um": 1.1, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 12, "KeepShutterOpenChannels": false, "Source": "Micro-Manager", "Channels": 4, "ComputerName": "ZadorConfocal2", "CustomIntervals_ms": [], "KeepShutterOpenSlices": true, "ChColors": [ -16711681, -256, -65281, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536 ], "Positions": 78, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\LiYuan\\06142019_65A\\Batch2" }, "Source": "PRIME", "PRIME-Name": "PMPCIECam00", "COM9-Name": "COM9", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "640 Shutter-Name": "AnalogIO", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "PRIME-PMode": "Normal", "COM4-DelayBetweenCharsMs": "0.0000", "TE2000-Port": "COM4", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PRIME-TransposeXY": "0", "640 AO-IOChannel": "Dev1/ao7", "555 AO-TriggerSequenceLength": "1000", "XLIGHT Emission Wheel-Label": "725/40 (C)", "COM3-Parity": "None", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "PRIME-TransposeCorrection": "0", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "Vincent-D3-Name": "Vincent-D3", "PRIME-PreampOffLimit": "10000", "PRIME-PP 4 MIN ADU AFFECTED": "200", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "Core-Galvo": "", "Position": "Default", "COM9-Handshaking": "Off", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "PIEZO", "COM1-Verbose": "1", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "PRIME-Exposure": "250.00", "405 AO-Demo": "No", "PRIME-PP 4 THRESHOLD": "75", "PRIME-CircularBufferAutoSize": "ON", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "PRIME-Description": "PVCAM API device adapter", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "TE2000-ModelType": "1", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "PRIME-AcquisitionMethod": "Callbacks", "PRIME-ShutterMode": "Pre-Exposure", "445 Shutter-SupportsTriggering": "0", "Core-XYStage": "XYStage", "PRIME-PP 2 ENABLED": "Yes", "XYStage-StepSize-X": "0.1000", "XYStage-StepSize-Y": "0.1000", "PRIME-Color": "OFF", "COM9-Description": "Serial port driver (boost:asio)", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao8", "Core-AutoFocus": "", "PRIME-Timing-ExposureTimeNs": "299990000.0000", "470 Shutter-Volts": "0.0000", "640 Shutter-Description": "NI DAC", "PIEZO-Description": "Ludl stage driver adapter", "640 AO-MinVolts": "0.0000", "BitDepth": 12, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "XLIGHT Hub-Port": "COM3", "TE2000-Version": "V2.50-V2.50-V2.10", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2350624, "640 Shutter-Sequenceable": "1", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "PRIME-Trigger-Expose Out-Mux": "1", "OpticalPath-State": "4", "PositionName": "EF65ASlide30R_Seq03_Contra_09", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "COM9-AnswerTimeout": "10000.0000", "520 Shutter-SupportsTriggering": "0", "Height": 1200, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "PRIME-Port": "CMOS", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "PRIME-SMARTStreamingEnabled": "No", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-Name": "AnalogIO", "PRIME-CircularBufferEnabled": "ON", "470 AO-Name": "AnalogIO", "PRIME-Timing-ReadoutTimeNs": "12336000.0000", "640 Shutter-MinVolts": "0.0000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "640 Shutter-TriggerSequenceLength": "1000", "PRIME-PP 2 THRESHOLD": "125", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "520 AO-Demo": "No", "PRIME-FTCapable": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Vincent-D3-Time to open (ms)": "35.0000", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "528 AO-Demo": "No", "445 Shutter-TriggerSequenceLength": "1000", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "528 Shutter-TriggerSampleRate": "100000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao6", "PRIME-ShutterOpenDelay": "0", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "405 Shutter-TriggerSequenceLength": "1000", "Focus-Position": "19907.0000", "COM1-AnswerTimeout": "500.0000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-Demo": "No", "640 AO-Name": "AnalogIO", "PRIME-Binning": "1x1", "470 AO-Sequenceable": "1", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "520 Shutter-MinVolts": "0.0000", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "XYStage-Name": "Stage", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "555 Shutter-Sequenceable": "1", "PRIME-Trigger Timeout (secs)": "10", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "PRIME-CCDTemperature": "-16.2700", "470 AO-IOChannel": "Dev1/ao2", "Focus-Description": "Nikon TE2000 focus adapter", "555-State": "0", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "XYStage-StartSpeed": "500.0000", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "640-DA Device": "640 Shutter", "PRIME-PVCAM Version": "3.7.1", "640 AO-SupportsTriggering": "0", "Vincent-D3-Channel #": "Ch. #1", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "5", "XYStage-Speed": "1800.0000", "528 AO-TriggerSampleRate": "100000", "405 Shutter-Demo": "No", "Focus-FocusStepSize_(nm)": "100", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "520 AO-MinVolts": "0.0000", "PixelSizeUm": 1.1, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "PRIME-ActualInterval-ms": "337.4000", "COM9-StopBits": "2", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "PRIME-ExposeOutMode": "First Row", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Vincent-D3-Command": "Close", "COM9-Verbose": "1", "470 Shutter-Name": "AnalogIO", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao11", "PRIME-SerialNumber": "A18B203008", "PRIME-PixelType": "12bit", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": -22.8, "XYStage-TransposeMirrorX": "0", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "4.0000", "555 Shutter-Description": "NI DAC", "PRIME-TriggerMode": "Internal Trigger", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 192.995, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "QUAD", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "TE2000-Description": "Nikon TE2000 hub", "FilterBlock-Label": "Confocal", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "PRIME-FirmwareVersion": "20.28", "XYStage-StepSize": "0.1000", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "PRIME-Gain": "1-Full well", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Timing-PreTriggerDelayNs": "12336000.0000", "Camera": "", "445 AO-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "PRIME-Timing-ClearingTimeNs": "24672000.0000", "PRIME-PreampDelay": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM1-DataBits": "8", "520 AO-IOChannel": "Dev1/ao4", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "0", "NextFrame": 0, "COM9-Parity": "None", "528-Description": "DA device that is used as a shutter", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "Time": "2019-06-16 01:03:28 -0400", "405 AO-IOChannel": "Dev1/ao0", "PRIME-FanSpeedSetpoint": "High", "COM4-Name": "COM4", "PRIME-ReadoutRate": "200MHz 12bit", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "PRIME-ShutterCloseDelay": "0", "PRIME-CircularBufferFrameCount": "46", "555 Shutter-Block voltage": "false", "PIEZO-StepSize": "0.0050", "445 AO-TriggerSequenceLength": "1000", "COM9-DataBits": "8", "520 AO-Block voltage": "false", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "445 AO-MinVolts": "0.0000", "PRIME-ClearCycles": "2", "PRIME-X-dimension": "1200", "Core-TimeoutMs": "5000", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "470 Shutter-TriggerSequenceLength": "1000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "555-Description": "DA device that is used as a shutter", "OpticalPath-Name": "OpticalPath", "Vincent-D3-Time to close (ms)": "35.0000", "Width": 1200, "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "445 AO-SupportsTriggering": "0", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "1", "PIEZO-Name": "Stage", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "640 AO-Volts": "5.0000", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao10", "Vincent-D3-Port": "COM1", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao13", "XYStage-Description": "Ludl XY stage driver adapter", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM9", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "OpticalPath-Label": "Left(100)", "YPositionUm": -47.9, "COM1-BaudRate": "9600", "445 AO-Name": "AnalogIO", "555 Shutter-Volts": "0.0000", "528 AO-IOChannel": "Dev1/ao5", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "PRIME-PVCAM Adapter Version": "1.3.29" } }